PCNA anticorps (AA 1-261)

N° du produit ABIN7599179

L’anticorps Lapin Polyclonal anti-PCNA a été validé pour WB, IHC, ELISA, IF, FACS et ICC. Il convient pour détecter PCNA dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PCNA anticorps (AA 1-261) (ABIN7599179)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-PCNA anticorps

Épitope: AA 1-261

Fonction: Anti-PCNA Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PCNA Antibody Picoband® (ABIN7599179). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PCNA recombinant protein (Position: M1-S261).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Moldovan GL, Pfander B, Jentsch S (May 18, 2007). "PCNA, the maestro of the replication fork". Cell. 129 (4): 665-79. 2. Warbrick E (Mar 1998). "PCNA binding through a conserved motif". BioEssays. 20 (3): 195-9. 3. Mailand N, Gibbs-Seymour I, Bekker-Jensen S (May 2013). "Regulation of PCNA-protein interactions for genome stability". Nature Reviews. Molecular Cell Biology. 14 (5): 269-82.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet:

Synonyms: Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

Background: Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Poids moléculaire: 36 kDa

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy