GRB10 anticorps (AA 1-276)

N° du produit ABIN7599204

Cet anticorps anti-GRB10 est un anticorps Lapin Polyclonal détectant GRB10 dans WB, ELISA, IHC et FACS. Adapté pour Souris et Rat.

Aperçu rapide pour GRB10 anticorps (AA 1-276) (ABIN7599204)

Antigène: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRB10 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-GRB10 anticorps

Épitope: AA 1-276

Fonction: Anti-GRB10 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-GRB10 Antibody Picoband® (ABIN7599204). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse GRB10 recombinant protein (Position: M1-K276).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Arnaud, P., Monk, D., Hitchins, M., Gordon, E., Dean, W., Beechey, C. V., Peters, J., Craigen, W., Preece, M., Stanier, P., Moore, G. E., Kelsey, G.Conserved methylation imprints in the human and mouse GRB10 genes with divergent allelic expression suggests differential reading of the same mark.Hum. Molec. Genet. 12: 1005-1019, 2003. 2. Charalambous, M., Smith, F. M., Bennett, W. R., Crew, T. E., Mackenzie, F., Ward, A.Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism.Proc. Nat. Acad. Sci. 100: 8292-8297, 2003. 3. Frantz, J. D., Giorgetti-Peraldi, S., Ottinger, E. A., Shoelson, S. E.Human GRB-IR-beta/GRB10: splice variants of an insulin and growth factor receptor-binding protein with PH and SH2 domains.J. Biol. Chem. 272: 2659-2667, 1997.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur GRB10

Antigène: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Autre désignation: Grb10

Sujet:

Synonyms: Deoxycytidine kinase, dCK, DCK,

Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Background: GRB10, Growth factor receptor-bound protein 10, also known as insulin receptor-binding protein Grb-IR is a protein that in humans is encoded by the GRB10 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors (e.g., IGF1R and IGF2R). Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified.

Poids moléculaire: 76 kDa

ID gène: 14783

UniProt: Q60760

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2