ME1 anticorps (AA 1-572)

N° du produit ABIN7599485

L’anticorps Souris Monoclonal anti-ME1 a été validé pour WB et IHC. Il convient pour détecter ME1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ME1 anticorps (AA 1-572) (ABIN7599485)

Antigène: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ME1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 5E5F7

Détail du produit anti-ME1 anticorps

Épitope: AA 1-572

Fonction: Anti-ME1 Antibody Picoband® (monoclonal, 5E5F7)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ME1 Antibody Picoband® (monoclonal, 5E5F7) (ABIN7599485). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human ME1 recombinant protein (Position: M1-Q572).

Isotype: IgG1

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
1. Chen, T.-R., McMorris, F. A., Creagan, R., Ricciuti, F. C., Tischfield, J., Ruddle, F. H. Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man. Am. J. Hum. Genet. 25: 200-207, 1973. 2. Cohen, P. T. W., Omenn, G. S. Genetic variation of the cytoplasmic and mitochondrial malic enzymes in the monkey: Macaca nemestrina. Biochem. Genet. 7: 289-301, 1972. 3. Gimelbrant, A., Hutchinson, J. N., Thompson, B. R., Chess, A. Widespread monoallelic expression on human autosomes. Science 318: 1136-1140, 2007.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur ME1

Antigène: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Autre désignation: ME1

Sujet:

Synonyms: Survival motor neuron protein, Component of gems 1, Gemin-1, SMN1, SMN, SMNT, SMN2, SMNC

Tissue Specificity: Expressed in a wide variety of tissues. Expressed at high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle, and low levels in fibroblasts and lymphocytes. Also seen at high levels in spinal cord. Present in osteoclasts and mononuclear cells (at protein level).

Background: NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Poids moléculaire: 64 kDa

ID gène: 4199

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha