SART1 anticorps (AA 125-800)

N° du produit ABIN7599884

Cet anticorps Lapin Polyclonal détecte spécifiquement SART1 dans WB, ELISA, IF, IHC, ICC et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour SART1 anticorps (AA 125-800) (ABIN7599884)

Antigène: SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SART1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-SART1 anticorps

Épitope: AA 125-800

Fonction: Anti-SART1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SART1 Antibody Picoband® (ABIN7599884). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SART1 recombinant protein (Position: K125-K800).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 3/1/2021. 2. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004. 3. Makarova, O. V., Makarov, E. M., Luhrmann, R. The 65 and 110 kDa SR-related proteins of the U4/U6-U5 tri-snRNP are essential for the assembly of mature spliceosomes. EMBO J. 20: 2553-2563, 2001.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur SART1

Antigène: SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))

Autre désignation: SART1

Sujet:

Synonyms: POU domain, class 4, transcription factor 1

Tissue Specificity: Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Background: U4/U6.U5 tri-snRNP-associated protein 1 is a protein that in humans is encoded by the SART1 gene. This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation, its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

Poids moléculaire: 110 kDa

ID gène: 9092

UniProt: O43290

Pathways: Ribonucleoprotein Complex Subunit Organization