C3 anticorps (AA 1277-1538)

N° du produit ABIN7599899

Cet anticorps Lapin Polyclonal détecte spécifiquement C3 dans WB, ELISA et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour C3 anticorps (AA 1277-1538) (ABIN7599899)

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp C3 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-C3 anticorps

Épitope: AA 1277-1538

Fonction: Anti-C3 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-C3 Antibody Picoband® (ABIN7599899). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human C3 recombinant protein (Position: Q1277-E1538). Human C3 shares 73.7 % and 76.3% amino acid (aa) sequence identity with mouse and rat C3, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ajees, A. A., Gunasekaran, K., Volanakis, J. E., Narayana, S. V. L., Kotwal, G. J., Krishna Murthy, H. M. The structure of complement C3b provides insights into complement activation and regulation. Nature 444: 221-225, 2006. Note: Retraction: Nature 532: 268 only, 2016. 2. Alper, C. A., Colten, H. R., Gear, J. S. S., Rabson, A. R., Rosen, F. S. Homozygous human C3 deficiency: the role of C3 in antibody production, C1s-induced vasopermeability, and cobra venom-induced passive hemolysis. J. Clin. Invest. 57: 222-229, 1976. 3. Alper, C. A., Colten, H. R., Rosen, S. F., Rabson, A. R., MacNab, G. M., Gear, J. S. S. Homozygous deficiency of C3 in a patient with repeated infections. Lancet 300: 1179-1181, 1972. Note: Originally Volume II.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur C3

Antigène: C3 (Complement Component 3 (C3))

Autre désignation: C3

Sujet:

Synonyms: Interleukin-17C, Il-17c, Cytokine CX2, Il17c

Tissue Specificity: Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes.

Background: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

Poids moléculaire: 128 kDa, 187 kDa

ID gène: 718

UniProt: P01024

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling