CENPE anticorps (AA 1390-2474)

N° du produit ABIN7600005

L’anticorps Lapin Polyclonal anti-CENPE a été validé pour WB et ELISA. Il convient pour détecter CENPE dans des échantillons de Souris et Rat.

Aperçu rapide pour CENPE anticorps (AA 1390-2474) (ABIN7600005)

Antigène: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CENPE est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-CENPE anticorps

Épitope: AA 1390-2474

Fonction: Anti-Cenpe Antibody Picoband®

Attributs du produit: Anti-Cenpe Antibody Picoband® (ABIN7600005). Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse Cenpe recombinant protein (Position: Q1390-Q2474). Mouse Cenpe shares 49.9% amino acid (aa) sequence identity with human Cenpe, respectively.

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Abrieu, A., Kahana, J. A., Wood, K. W., Cleveland, D. W. CENP-E as an essential component of the mitotic checkpoint in vitro. Cell 102: 817-826, 2000. 2. Barisic, M., Silva e Sousa, R., Tripathy, S. K., Magiera, M. M., Zaytsev, A. V., Pereira, A. L., Janke, C., Grishchuk, E. L., Maiato, H. Microtubule detyrosination guides chromosomes during mitosis. Science 348: 799-803, 2015. 3. Gross, M. B. Personal Communication. Baltimore, Md. 11/4/2014.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur CENPE

Antigène: CENPE (Centromere Protein E, 312kDa (CENPE))

Autre désignation: Cenpe

Sujet: Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

Poids moléculaire: 300 kDa

ID gène: 229841

Pathways: M Phase, Maintenance of Protein Location