SIRT1 anticorps (AA 142-533)

N° du produit ABIN7600052

Cet anticorps Lapin Polyclonal détecte spécifiquement SIRT1 dans WB, ELISA, IF et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour SIRT1 anticorps (AA 142-533) (ABIN7600052)

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIRT1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-SIRT1 anticorps

Épitope: AA 142-533

Fonction: Anti-SIRT1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SIRT1 Antibody Picoband® (ABIN7600052). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SIRT1 recombinant protein (Position: Y142-H533).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5μ,g/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Wilson BJ, Tremblay AM, Deblois G, Sylvain-Drolet G, Giguère V (Jul 2010). "An acetylation switch modulates the transcriptional activity of estrogen-related receptor alpha.". Mol. Endocrinol. 24 (7): 1349-58. 2. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P (Mar 2005). "Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1". Nature 434 (7029): 113-8. 3. Nemoto S, Fergusson MM, Finkel T (Apr 2005). "SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1 alpha". J Biol Chem. 280 (16): 16456-60.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur SIRT1

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Autre désignation: SIRT1

Sujet:

Synonyms: NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT1, SIR2L1

Tissue Specificity: Widely expressed.

Background: Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

Poids moléculaire: 120 kDa

ID gène: 23411

Pathways: Signalisation MAPK, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling