NSUN2 anticorps (AA 146-654)

N° du produit ABIN7600077

L’anticorps Lapin Polyclonal anti-NSUN2 a été validé pour WB, ELISA, IHC, IF, ICC et FACS. Il convient pour détecter NSUN2 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NSUN2 anticorps (AA 146-654) (ABIN7600077)

Antigène: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSUN2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-NSUN2 anticorps

Épitope: AA 146-654

Fonction: Anti-NSUN2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-NSUN2 Antibody Picoband® (ABIN7600077). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human NSUN2 recombinant protein (Position: H146-K654).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abbasi-Moheb, L., Mertel, S., Gonsior, M., Nouri-Vahid, L., Kahrizi, K., Cirak, S., Wieczorek, D., Motazacker, M. M., Esmaeeli-Nieh, S., Cremer, K., Weissmann, R., Tzschach, A., Garshasbi, M., Abedini, S. S., Najmabadi, H., Ropers, H. H., Sigrist, S. J., Kuss, A. W. Mutations in NSUN2 cause autosomal-recessive intellectual disability. Am. J. Hum. Genet. 90: 847-855, 2012. 2. Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S., Frye, M. Stem cell function and stress response are controlled by protein synthesis. Nature 534: 335-340, 2016. 3. Blanco, S., Dietmann, S., Flores, J. V., Hussain, S., Kutter, C., Humphreys, P., Lukk, M., Lombard, P., Treps, L., Popis, M., Kellner, S., Holter, S. M., and 13 others. l Aberrant methylation of tRNAs links cellular stress to neuro-developmental disorders. EMBO J. 33: 2020-2039, 2014.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur NSUN2

Antigène: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Autre désignation: NSUN2

Sujet:

Synonyms: Transient receptor potential cation channel subfamily V member 3, TrpV3, Vanilloid receptor-like 3, VRL-3, TRPV3

Tissue Specificity: Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion. Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles.

Background: NOP2/Sun domain family, member 2 is a protein that in humans is encoded by the NSUN2 gene. This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Poids moléculaire: 100 kDa

ID gène: 54888

UniProt: Q08J23