HNF4A anticorps (AA 164-474)

N° du produit ABIN7600215

Cet anticorps Souris Monoclonal détecte spécifiquement HNF4A dans WB et IHC. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour HNF4A anticorps (AA 164-474) (ABIN7600215)

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HNF4A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 6C8E9

Détail du produit anti-HNF4A anticorps

Épitope: AA 164-474

Fonction: Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9) (ABIN7600215). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequence identity with mouse and rat HNF-4-alpha, respectively.

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
1. Chartier FL, Bossu JP, Laudet V, Fruchart JC, Laine B (September 1994). "Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver". Gene 147 (2): 269-72. 2. Argyrokastritis A, Kamakari S, Kapsetaki M, Kritis A, Talianidis I, Moschonas NK (February 1997). "Human hepatocyte nuclear factor-4 (hHNF-4) gene maps to 20q12-q13.1 between PLCG1 and D20S17". Hum. Genet. 99 (2): 233-6. 3. Tirona RG, Lee W, Leake BF, Lan LB, Cline CB, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, Schuetz EG, Kim RB (February 2003). "The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4". Nat. Med. 9 (2): 220-4.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur HNF4A

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Autre désignation: HNF4A

Sujet:

Synonyms: AHUS2 antibody, Antigen defined by monoclonal antibody, TRA 2 10 antibody, Antigen identified by monoclonal antibody, TRA 2 10 antibody, CD46 antibody, CD46 antigen antibody, CD46 antigen complement regulatory protein antibody, CD46 Molecule antibody, CD46 Molecule complement regulatory protein antibody, Complement membrane cofactor protein antibody, MCP antibody, MCP_HUMAN antibody, Measles virus receptor antibody, membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody, Membrane cofactor protein antibody, MGC26544 antibody,MIC10 antibody, TLX antibody, TRA2.10 antibody, Trophoblast leucocyte common antigen antibody, Trophoblast leukocyte common antigen antibody, Trophoblast lymphocyte cross reactive antigen antibody

Tissue Specificity: Expressed by all cells except erythrocytes.

Background: Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Poids moléculaire: 53 kDa

ID gène: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process