PPM1F anticorps (AA 17-454)

N° du produit ABIN7600269

Cet anticorps Lapin Polyclonal détecte spécifiquement PPM1F dans WB, ELISA, IF et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour PPM1F anticorps (AA 17-454) (ABIN7600269)

Antigène: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPM1F est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PPM1F anticorps

Épitope: AA 17-454

Fonction: Anti-PPM1F Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PPM1F Antibody Picoband® (ABIN7600269). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PPM1F recombinant protein (Position: E17-S454).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 4/30/2021. 2. Ishida, A., Sueyoshi, N., Kameshita, I. Functions and dysfunctions of Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E. Arch. Biochem. Biophys. 640: 83-92, 2018. 3. Kim, P. R., Koon, Y. L., Lee, R. T. C., Azizan, F., Koh, D. H. Z., Chiam, K.-H., Koh, C.-G. Phosphatase POPX2 interferes with cell cycle by interacting with Chk1. Cell Cycle 19: 405-418, 2020.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PPM1F

Antigène: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Autre désignation: PPM1F

Sujet:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Poids moléculaire: 54 kDa

ID gène: 9647

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity