SKP1 anticorps (AA 2-128)

N° du produit ABIN7600465

L’anticorps Lapin Polyclonal anti-SKP1 a été validé pour WB, ELISA et IHC. Il convient pour détecter SKP1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SKP1 anticorps (AA 2-128) (ABIN7600465)

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-SKP1 anticorps

Épitope: AA 2-128

Fonction: Anti-SKP1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SKP1 Antibody Picoband® (ABIN7600465). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SKP1 recombinant protein (Position: P2-K128).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arai, T., Kasper, J. S., Skaar, J. R., Ali, S. H., Takahashi, C., DeCaprio, J. A. Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc. Nat. Acad. Sci. 100: 9855-9860, 2003. 2. Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J. W., Elledge, S. J. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86: 263-274, 1996. 3. Chen, H., Thalmann, I., Adams, J. C., Avraham, K. B., Copeland, N. G., Jenkins, N. A., Beier, D. R., Corey, D. P., Thalmann, R., Duyk, G. M. cDNA cloning, tissue distribution and chromosomal localization of Ocp2, a gene encoding a putative transcription-associated factor predominantly expressed in the auditory organs. Genomics 27: 389-398, 1995.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur SKP1

Antigène: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Autre désignation: SKP1

Sujet:

Synonyms: S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, P19a, Organ of Corti protein 2, OCP-2, Organ of Corti protein II, OCP-II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, SKP1, EMC19, OCP2, SKP1A, TCEB1L

Tissue Specificity: Ubiquitously expressed.

Background: Skp1 (S-phase kinase-associated protein 1) is a small protein of approximately 160 amino acids. It is mapped to 5q31.1. This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds ly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein (s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Poids moléculaire: 19 kDa

ID gène: 6500

UniProt: P63208

Pathways: Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases