RNASEH2A anticorps (AA 25-299)

N° du produit ABIN7600899

Cet anticorps Lapin Polyclonal détecte spécifiquement RNASEH2A dans WB, ELISA et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour RNASEH2A anticorps (AA 25-299) (ABIN7600899)

Antigène: RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNASEH2A est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-RNASEH2A anticorps

Épitope: AA 25-299

Fonction: Anti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband® (ABIN7600899). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Ribonuclease H2, subunit A/RNASEH2A recombinant protein (Position: R25-L299).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Coffin, S. R., Hollis, T., Perrino, F. W. Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome. J. Biol. Chem. 286: 16984-16991, 2011. 2. Crow, Y. J., Leitch, A., Hayward, B. E., Garner, A., Parmar, R., Griffith, E., Ali, M., Semple, C., Aicardi, J., Babul-Hirji, R., Baumann, C., Baxter, P., and 33 others. Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nature Genet. 38: 910-916, 2006. 3. Frank, P., Braunshofer-Reiter, C., Wintersberger, U., Grimm, R., Busen, W. Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII. Proc. Nat. Acad. Sci. 95: 12872-12877, 1998.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur RNASEH2A

Antigène: RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))

Autre désignation: RNASEH2A

Sujet:

Synonyms: Mucin-20, MUC-20, MUC20, KIAA1359, UNQ2782/PRO7170

Tissue Specificity: Highly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.

Background: Ribonuclease H2 subunit A, also known as RNase H2 subunit A, is an enzyme that in humans is encoded by the RNASEH2A gene. The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.

Poids moléculaire: 33 kDa

ID gène: 10535

UniProt: O75792