DNMT3B anticorps (AA 271-853)

N° du produit ABIN7601041

L’anticorps Lapin Polyclonal anti-DNMT3B a été validé pour WB, ELISA et FACS. Il convient pour détecter DNMT3B dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour DNMT3B anticorps (AA 271-853) (ABIN7601041)

Antigène: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT3B est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-DNMT3B anticorps

Épitope: AA 271-853

Fonction: Anti-DNMT3B Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-DNMT3B Antibody Picoband® (ABIN7601041). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DNMT3B recombinant protein (Position: E271-E853).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Balada, E., Ordi-Ros, J., Serrano-Acedo, S., Martinez-Lostao, L., Rosa-Leyva, M., Vilardell-Tarres, M. Transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus. Immunology 124: 339-347, 2008. 2. Barau, J., Teissandier, A., Zamudio, N., Roy, S., Nalesso, V., Herault, Y., Guillou, F., Bourc'his, D. The DNA methyltransferase DNMT3C protects male germ cells from transposon activity. Science 354: 909-912, 2016. 3. Beaulieu, N., Morin, S., Chute, I. C., Robert, M.-F., Nguyen, H., MacLeod, A. R. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. J. Biol. Chem. 277: 28176-28181, 2002.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur DNMT3B

Antigène: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Autre désignation: DNMT3B

Sujet:

Synonyms: Copper chaperone for superoxide dismutase,Superoxide dismutase copper chaperone,CCS,

Tissue Specificity: Ubiquitous.

Background: DNA (cytosine-5-)-methyltransferase 3 beta, is an enzyme that in humans in encoded by the DNMT3B gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Poids moléculaire: 100 kDa

ID gène: 1789

UniProt: P50570