Nicastrin anticorps (AA 34-669)

N° du produit ABIN7601402

Cet anticorps anti-Nicastrin est un anticorps Lapin Polyclonal détectant Nicastrin dans WB, ELISA, IF et ICC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour Nicastrin anticorps (AA 34-669) (ABIN7601402)

Antigène: Nicastrin (NCSTN)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nicastrin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Nicastrin anticorps

Épitope: AA 34-669

Fonction: Anti-Nicastrin/NCSTN Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-heavy chain Nicastrin/NCSTN Antibody Picoband® (ABIN7601402). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Nicastrin/NCSTN recombinant protein (Position: N34-E669).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: NCSTN nicastrin" 2. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N (October 1996)."Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Res. 3 (5): 321-9, 341-54. 3. Zhang YW, Luo WJ, Wang H, Lin P, Vetrivel KS, Liao F, Li F, Wong PC, Farquhar MG, Thinakaran G, Xu H (April 2005)."Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components".J. Biol. Chem. 280 (17): 17020-6.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Nicastrin

Antigène: Nicastrin (NCSTN)

Autre désignation: NCSTN

Sujet:

Synonyms: Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65

Tissue Specificity: Detected in thymus and in activated splenocytes.

Background: Nicastrin, also known asNCSTN, is aproteinthat is a part of the gamma secretaseprotein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretaseprotein complex, all of which undergo significant post-translational modificationbefore becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex.The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly.The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin has also been identified as a regulator of neprilysin, anenzymeinvolved in the degradation of amyloid beta fragment.

Poids moléculaire: 120 kDa

ID gène: 23385

UniProt: Q92542

Pathways: Signalisation Notch, Neurotrophin Signaling Pathway