MCM2 anticorps (AA 393-850)

N° du produit ABIN7601594

Cet anticorps anti-MCM2 est un anticorps Monoclonal produit chez le Souris, détectant MCM2 dans WB, IHC, IF, ICC et FACS. Convient pour Humain.

Aperçu rapide pour MCM2 anticorps (AA 393-850) (ABIN7601594)

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 11C4

Détail du produit anti-MCM2 anticorps

Épitope: AA 393-850

Fonction: Anti-MCM2 Antibody (monoclonal, 11C4)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MCM2 Antibody (monoclonal, 11C4) (ABIN7601594). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MCM2 recombinant protein (Position: S393-R850).

Isotype: IgG1

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MCM2

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Autre désignation: MCM2

Sujet:

Synonyms: Elafin, Elastase-specific inhibitor, ESI, Peptidase inhibitor 3, PI-3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, SKALP, WAP four-disulfide core domain protein 14, PI3, WAP3, WFDC14

Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Background: MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2), also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Poids moléculaire: 125 kDa

ID gène: 4171

UniProt: P49736

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA