Glutaminase anticorps (AA 396-654)

N° du produit ABIN7601602

L’anticorps Souris Monoclonal anti-Glutaminase a été validé pour WB, IHC, IF et ICC. Il convient pour détecter Glutaminase dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Glutaminase anticorps (AA 396-654) (ABIN7601602)

Antigène: Glutaminase (GLS)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Glutaminase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 9G6

Détail du produit anti-Glutaminase anticorps

Épitope: AA 396-654

Fonction: Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 9G6)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 9G6) (ABIN7601602). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Lynch, D. S., Chelban, V., Vandrovcova, J., Pittman, A., Wood, N. W., Houlden, H. GLS loss of function causes autosomal recessive spastic ataxia and optic atrophy. Ann. Clin. Transl. Neurol. 5: 216-221, 2018. 2. Modi, W. S., Pollock, D. D., Mock, B. A., Banner, C., Renauld, J.-C., Van Snick, J. Regional localization of the human glutaminase (GLS) and interleukin-9 (IL9) genes by in situ hybridization. Cytogenet. Cell Genet. 57: 114-116, 1991. 3. Rumping, L., Tessadori, F., Pouwels, P. J. W., Vringer, E., Wijnen, J. P., Bhogal, A. A., Savelberg, S. M. C., Duran, K. J., Bakkers, M. J. G., Ramos, R. J. J., Schellekens, P. A. W., Kroes, H. Y., and 16 others. GLS hyperactivity causes glutamate excess, infantile cataract and profound developmental delay. Hum. Molec. Genet. 28: 96-104, 2019.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Glutaminase

Antigène: Glutaminase (GLS)

Autre désignation: GLS

Sujet:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

Tissue Specificity: Widely expressed. Overexpressed in prostate cancer.

Background: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

Poids moléculaire: 56-73 kDa

ID gène: 2744

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, L'effet Warburg