ChT anticorps (AA 446-580)

N° du produit ABIN7601754

Cet anticorps Lapin Polyclonal détecte spécifiquement ChT dans WB, FACS et ELISA. Il présente une réactivité envers Souris, Humain et Rat.

Aperçu rapide pour ChT anticorps (AA 446-580) (ABIN7601754)

Antigène: ChT (High Affinity Choline Transporter (ChT))

Reactivité: Souris, Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ChT est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Détail du produit anti-ChT anticorps

Épitope: AA 446-580

Fonction: Anti-SLC5A7 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SLC5A7 Antibody Picoband® (ABIN7601754). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Apparsundaram, S., Ferguson, S. M., George, A. L., Jr., Blakely, R. D. Molecular cloning of a human, hemicholinium-3-sensitive choline transporter. Biochem. Biophys. Res. Commun. 276: 862-867, 2000. 2. Barwick, K. E. S., Wright, J., Al-Turki, S., McEntagart, M. M., Nair, A., Chioza, B., Al-Memar, A., Modarres, H., Reilly, M. M., Dick, K. J., Ruggiero, A. M., Blakely, R. D., Hurles, M. E., Crosby, A. H. Defective presynaptic choline transport underlies hereditary motor neuropathy. Am. J. Hum. Genet. 91: 1103-1107, 2012. 3. Bauche, S., O'Regan, S., Azuma, Y., Laffargue, F., McMacken, G., Sternberg, D., Brochier, G., Buon, C., Bouzidi, N., Topf, A., Lacene, E., Remerand, G., and 30 others. Impaired presynaptic high-affinity choline transporter causes a congenital myasthenic syndrome with episodic apnea. Am. J. Hum. Genet. 99: 753-761, 2016.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur ChT

Antigène: ChT (High Affinity Choline Transporter (ChT))

Autre désignation: SLC5A7

Sujet:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: The high-affinity choline transporter (ChT) also known as solute carrier family 5 member 7 is a protein in humans that is encoded by the SLC5A7 gene. This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 80 kDa

ID gène: 60482