SYVN1 anticorps (AA 68-604)

N° du produit ABIN7602291

Cet anticorps Lapin Polyclonal détecte spécifiquement SYVN1 dans WB, ELISA, FACS, IF et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour SYVN1 anticorps (AA 68-604) (ABIN7602291)

Antigène: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SYVN1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-SYVN1 anticorps

Épitope: AA 68-604

Fonction: Anti-HRD1/SYVN1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-HRD1/SYVN1 Antibody Picoband® (ABIN7602291). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human HRD1/SYVN1 recombinant protein (Position: Q68-R604).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Amano, T., Yamasaki, S., Yagishita, N., Tsuchimochi, K., Shin, H., Kawahara, K., Aratani, S., Fujita, H., Zhang, L., Ikeda, R., Fujii, R., Miura, N., Komiya, S., Nishioka, K., Maruyama, I., Fukamizu, A., Nakajima, T. Synoviolin/Hrd1, an E3 ubiquitin ligase, as a novel pathogenic factor for arthropathy. Genes Dev. 17: 2436-2449, 2003. 2. Burr, M. L., Cano, F., Svobodova, S., Boyle, L. H., Boname, J. M., Lehner, P. J. HRD1 and UBE2J1 target misfolded MHC class I heavy chains for endoplasmic reticulum-associated degradation. Proc. Nat. Acad. Sci. 108: 2034-2039, 2011. 3. Kaneko, M., Ishiguro, M., Niinuma, Y., Uesugi, M., Nomura, Y. Human HRD1 protects against ER stress-induced apoptosis through ER-associated degradation. FEBS Lett. 532: 147-152, 2002.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur SYVN1

Antigène: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Autre désignation: SYVN1

Sujet:

Synonyms: Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

Tissue Specificity: Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Background: E3 ubiquitin-protein ligase synoviolin is an enzyme that in humans is encoded by the SYVN1 gene. This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms.

Poids moléculaire: 76 kDa

ID gène: 84447

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling