ALKBH1 anticorps (C-Term)

N° du produit ABIN7602742

L’anticorps Lapin Polyclonal anti-ALKBH1 a été validé pour WB, IHC, IF, ICC et FACS. Il convient pour détecter ALKBH1 dans des échantillons de Humain.

Aperçu rapide pour ALKBH1 anticorps (C-Term) (ABIN7602742)

Antigène: ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ALKBH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-ALKBH1 anticorps

Épitope: C-Term

Fonction: Anti-ALKBH1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ALKBH1 Antibody (ABIN7602742). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human ALKBH1, which shares 81.3% and 84.4% amino acid (aa) sequence identity with mouse and rat ALKBH1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Haag, S., Sloan, K. E., Ranjan, N., Warda, A. S., Kretschmer, J., Blessing, C., Hubner, B., Seikowski, J., Dennerlein, S., Rehling, P., Rodnina, M., Hobartner, C., Bohnsack, M. T. NSUN3 and ABH1 modify the wobble position of mt-tRNA(Met) to expand codon recognition in mitochondrial translation. EMBO J. 35: 2104-2119, 2016. 2. Tsujikawa, K., Koike, K., Kitae, K., Shinkawa, A., Arima, H., Suzuki, T., Tsuchiya, M., Makino, Y., Furukawa, T., Konishi, N., Yamamoto, H. Expression and sub-cellular localization of human ABH family molecules. J. Cell. Molec. Med. 11: 1105-1116, 2007. 3. Wei, Y. F., Carter, K. C., Wang, R. P., Shell, B. K. Molecular cloning and functional analysis of a human cDNA encoding an Escherichia coli AlkB homolog, a protein involved in DNA alkylation damage repair. Nucleic Acids Res. 24: 931-937, 1996.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ALKBH1

Antigène: ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))

Autre désignation: ALKBH1

Sujet:

Synonyms: Nucleic acid dioxygenase ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, mRNA N (3)-methylcytidine demethylase, ALKBH1, ABH, ABH1, ALKBH

Tissue Specificity: Ubiquitous.

Background: Nucleic acid dioxygenase ALKBH1 is an enzyme that in humans is encoded by the ALKBH1 gene. It is mapped to 14q24.3. This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.

Poids moléculaire: 43 kDa

ID gène: 8846

UniProt: Q13686

Pathways: Réparation de l'ADN