Recombinant Cadherin-16 anticorps (CF®740)

N° du produit ABIN7650259

Cet anticorps Souris Monoclonal détecte spécifiquement Cadherin-16 dans IHC, WB, FACS et IHC (fp). Il présente une réactivité envers Humain, Souris, Rat, Chien et Lapin.

Aperçu rapide pour Recombinant Cadherin-16 anticorps (CF®740) (ABIN7650259)

Antigène: Cadherin-16 (CDH16)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Chien, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cadherin-16 est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCDH16-1071

Détail du produit anti-Cadherin-16 anticorps (CF®740)

Fonction: Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 (rCDH16/1071), CF740 conjugate

Attributs du produit: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human CDH16 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Normal kidney or renal cell carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cadherin-16

Antigène: Cadherin-16 (CDH16)

Autre désignation: CDH16

Sujet:

Synonyms: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody

Gene Symbol: CDH16

Tissue Expression: Epithelial cells

Poids moléculaire: 130 kDa

ID gène: 1014

UniProt: O75309