CD63 anticorps (AA 100-197) (CF®488A)

N° du produit ABIN7651790

L’anticorps Souris Monoclonal anti-CD63 a été validé pour WB, IHC et IHC (fp). Il convient pour détecter CD63 dans des échantillons de Humain.

Aperçu rapide pour CD63 anticorps (AA 100-197) (CF®488A) (ABIN7651790)

Antigène: CD63

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est conjugé à/à la CF®488A

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: LAMP3-2790

Détail du produit anti-CD63 anticorps (CF®488A)

Épitope: AA 100-197

Fonction: CD63 (Late Endosomes Marker)(LAMP3/2790), CF488A conjugate

Attributs du produit: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment (around aa 100-197) of human CD63 protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: U87MG, SK-MEL-28, HL60, THP-1, NIH/3T3, or MCF-7 cells. Human melanoma, spleen or lymphoma tissue.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD63

Antigène: CD63

Autre désignation: CD63

Sujet:

Synonyms: gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30

Gene Symbol: CD63

Tissue Expression: Platelets|Granulocytes|Lymphocytes|Monocytes/macrophages

Poids moléculaire: 26 kDa (core protein), 30-60 kDa (glycosylated)

ID gène: 967

UniProt: P08962