Cyclin A1 anticorps (CF®640R)

N° du produit ABIN7652711

L’anticorps Souris Monoclonal anti-Cyclin A1 a été validé pour WB. Il convient pour détecter Cyclin A1 dans des échantillons de Xenopus laevis.

Aperçu rapide pour Cyclin A1 anticorps (CF®640R) (ABIN7652711)

Antigène: Cyclin A1 (CCNA1)

Reactivité: Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A1 est conjugé à/à la CF®640R

Application: Western Blotting (WB)

Clone: XLA1-3

Détail du produit anti-Cyclin A1 anticorps (CF®640R)

Fonction: Cyclin A1(XLA1-3), CF640R conjugate

Attributs du produit: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length Xenopus laevis Cyclin A1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Commentaires:

Positive Control: Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cyclin A1

Antigène: Cyclin A1 (CCNA1)

Autre désignation: Cyclin A1

Sujet:

Synonyms: CCN1, CCNA, CCNA2, Cyclin A1

Gene Symbol: ccna1

Poids moléculaire: 65 kDa

UniProt: P18606

Pathways: Apoptose, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA