Cyclin B2 anticorps (CF®594)

N° du produit ABIN7652749

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin B2 dans IF. Il présente une réactivité envers Humain, Souris, Rat et Xenopus laevis.

Aperçu rapide pour Cyclin B2 anticorps (CF®594) (ABIN7652749)

Antigène: Cyclin B2 (CCNB2)

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B2 est conjugé à/à la CF®594

Application: Immunofluorescence (IF)

Clone: X29-2

Détail du produit anti-Cyclin B2 anticorps (CF®594)

Fonction: Cyclin B2(X29.2), CF594 conjugate

Attributs du produit: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: ELISA: For coating, order antibody without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Commentaires:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cyclin B2

Antigène: Cyclin B2 (CCNB2)

Autre désignation: Cyclin B2

Sujet:

Synonyms: ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694

Gene Symbol: CCNB2

Poids moléculaire: 51 kDa

ID gène: 9133

UniProt: O95067

Pathways: Cycle Cellulaire, M Phase