Cytochrome C anticorps (CF®405S)

N° du produit ABIN7652953

Cet anticorps Souris Monoclonal détecte spécifiquement Cytochrome C dans FACS et IF. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour Cytochrome C anticorps (CF®405S) (ABIN7652953)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est conjugé à/à la CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6H2-B4

Détail du produit anti-Cytochrome C anticorps (CF®405S)

Fonction: Cytochrome C (Mitochondrial Marker)(6H2.B4), CF405S conjugate

Attributs du produit: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Immunogène: Rat full-length cytochrome c protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome c

Sujet:

Synonyms: CYC, CYCS, HCS, THC4

Gene Symbol: CYCS

Poids moléculaire: 15 kDa

ID gène: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity