Recombinant Cytochrome C anticorps (Biotin)

N° du produit ABIN7652992

Cet anticorps Lapin Monoclonal détecte spécifiquement Cytochrome C dans WB, IHC, FACS, IF et IHC (fp). Il présente une réactivité envers Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster et Grenouille.

Aperçu rapide pour Recombinant Cytochrome C anticorps (Biotin) (ABIN7652992)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster, Grenouille

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CYCS-3128R

Détail du produit anti-Cytochrome C anticorps (Biotin)

Fonction: Cytochrome C (Mitochondrial Marker)(CYCS/3128R), Biotin conjugate

Attributs du produit: Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e. cytochrome in the mitochondria with heme attached). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human CYCS protein

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome c

Sujet:

Synonyms: CYC, CYCS, HCS, THC4

Gene Symbol: CYCS

Poids moléculaire: 15 kDa

ID gène: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity