RAD51 anticorps (AA 1-134) (CF®405S)

N° du produit ABIN7653218

L’anticorps Souris Monoclonal anti-RAD51 a été validé pour WB et FACS. Il convient pour détecter RAD51 dans des échantillons de Humain.

Aperçu rapide pour RAD51 anticorps (AA 1-134) (CF®405S) (ABIN7653218)

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD51 est conjugé à/à la CF®405S

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: RAD51-2701

Détail du produit anti-RAD51 anticorps (CF®405S)

Épitope: AA 1-134

Fonction: RAD51 (Prognostic and Response to Chemotherapy Marker)(RAD51/2701), CF405S conjugate

Attributs du produit: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies. RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment of human RAD51 protein (around aa 1-134)

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: K562, HeLa or MCF-7 cells. Colon or breast Carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur RAD51

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Autre désignation: RAD51

Sujet:

Synonyms: RCA1/BRCA2 containing complex, subunit 5, BRCC5, DNA repair protein RAD51, FANCR, HsT16930, MRMV2, RAD51, RAD51 recombinase, RAD51A, RECA, RecA like protein, Recombination protein A

Gene Symbol: RAD51

Poids moléculaire: 37 kDa

ID gène: 5888

UniProt: Q06609

Pathways: Réparation de l'ADN