EGLN1 anticorps (AA 1-24)

N° du produit ABIN7653373

Cet anticorps Souris Monoclonal détecte spécifiquement EGLN1 dans . Il présente une réactivité envers Humain.

Aperçu rapide pour EGLN1 anticorps (AA 1-24) (ABIN7653373)

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGLN1 est non-conjugé

Application: Veuillez nous consulter SVP

Clone: 366G-76-3

Détail du produit anti-EGLN1 anticorps

Épitope: AA 1-24

Fonction: EGLN1 / PHD2(366G/76/3)

Attributs du produit: This antibody recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Residues 1-24 of PHD2

Isotype: IgG1, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: MCF7 cells. Brain or adrenal gland.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur EGLN1

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Autre désignation: EGLN1

Sujet:

Synonyms: Chromosome 1 Open Reading Frame 12 (C1ORF12), DKFZp761F179, ECYT3, Egl 9 family hypoxia inducible factor 1, Egl nine homolog 1, Egln1, HIF Prolyl Hydroxylase 2 (HIF PH2), HIFPH2, HPH2, Hypoxia inducible factor prolyl hydroxylase 2, ORF13, P4H2, PhD2, PNAS 118, PNAS 137, Prolyl hydroxylase domain-containing protein 2, SM20, Zinc finger MYND domain containing protein 6 (ZMYND6)

Gene Symbol: EGLN1

Poids moléculaire: 46 kDa

ID gène: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, L'effet Warburg