MCAM anticorps (CF®740)

N° du produit ABIN7656789

Cet anticorps anti-MCAM est un anticorps Souris Monoclonal détectant MCAM dans FACS, IF et ELISA. Adapté pour Humain.

Aperçu rapide pour MCAM anticorps (CF®740) (ABIN7656789)

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCAM est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Clone: OJ79c

Détail du produit anti-MCAM anticorps (CF®740)

Fonction: MUC18 / Mucin 18 / CD146(OJ79c), CF740 conjugate

Attributs du produit: The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human MUC18 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MCAM

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Autre désignation: CD146

Sujet:

Synonyms: Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1

Gene Symbol: MCAM

Poids moléculaire: 130 kDa

ID gène: 4162

UniProt: P43121