Recombinant ADRP anticorps (AA 249-376) (CF®647)

N° du produit ABIN7658555

L’anticorps Lapin Monoclonal anti-ADRP a été validé pour WB, IHC et IHC (fp). Il convient pour détecter ADRP dans des échantillons de Humain.

Aperçu rapide pour Recombinant ADRP anticorps (AA 249-376) (CF®647) (ABIN7658555)

Antigène: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ADRP est conjugé à/à la CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ADFP-2755R

Détail du produit anti-ADRP anticorps (CF®647)

Épitope: AA 249-376

Fonction: Adipophilin / Perilipin-2 (Marker of Lipid Accumulation) (ADFP/2755R), CF647 conjugate

Attributs du produit: Perilipin-2, also known as Adipophilin, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This 48 kDa protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur ADRP

Antigène: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Autre désignation: Adipophilin

Sujet:

Synonyms: Perilipin-2, Adipophilin, ADFP, ADRP, Adipose differentiation-related protein, Perilipin-2

Gene Symbol: PLIN2

Poids moléculaire: 48 kDa

ID gène: 123

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism