PODXL anticorps (Extracellular Domain) (CF®740)

N° du produit ABIN7658743

Cet anticorps Souris Monoclonal détecte spécifiquement PODXL dans WB, IHC, FACS, IF et IHC (fp). Il présente une réactivité envers Humain, Rat et Lapin.

Aperçu rapide pour PODXL anticorps (Extracellular Domain) (CF®740) (ABIN7658743)

Antigène: PODXL (Podocalyxin-Like (PODXL))

Reactivité: Humain, Rat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PODXL est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 3D3

Détail du produit anti-PODXL anticorps (CF®740)

Épitope: Extracellular Domain

Fonction: Podocalyxin(3D3), CF740 conjugate

Attributs du produit: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is highly on kidney podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Immunoprecipitation 1-2 μg/500 μg protein. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur PODXL

Antigène: PODXL (Podocalyxin-Like (PODXL))

Autre désignation: Podocalyxin

Sujet:

Synonyms: PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1

Gene Symbol: PODXL

Tissue Expression: Kidney

Poids moléculaire: 165-170 kDa

ID gène: 5420

UniProt: O00592

Pathways: Tube Formation