PCNA anticorps (CF®740)

N° du produit ABIN7659196

Cet anticorps Souris Monoclonal détecte spécifiquement PCNA dans IHC, FACS et IHC (fp). Il présente une réactivité envers Humain, Souris, Rat, Poulet, Singe, Porc, Saccharomyces cerevisiae, Poisson zèbre (Danio rerio), Drosophila melanogaster et Schizosaccharomyces pombe.

Aperçu rapide pour PCNA anticorps (CF®740) (ABIN7659196)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Porc, Saccharomyces cerevisiae, Poisson zèbre (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PC10

Détail du produit anti-PCNA anticorps (CF®740)

Fonction: PCNA(PC10), CF740 conjugate

Attributs du produit: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Rat PCNA/Protein A fusion protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Tonsil or reactive lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet:

Synonyms: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

Gene Symbol: PCNA

Tissue Expression: Proliferating cells

Poids moléculaire: 36 kDa

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy