Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) anticorps (CF®488A)

N° du produit ABIN7660201

Cet anticorps Souris Monoclonal détecte spécifiquement dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) anticorps (CF®488A) (ABIN7660201)

Antigène: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: CF®488A

Application: Western Blotting (WB)

Clone: RSPTBN2-1778

Détail du produit

Épitope: AA 356-475

Fonction: Spectrin beta III (SPTBN2)(rSPTBN2/1778), CF488A conjugate

Attributs du produit: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment (around aa356-475) of human SPTBN2 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: ELISA: Use Ab at 2-4 μg/mL for coating, Order Ab without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Commentaires:

Positive Control: PANC-1, HeLa, PC-3 cells. Human brain, pancreas or liver tissues (IHC).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détail du antigène

Antigène: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Autre désignation: Spectrin Beta-III

Sujet:

Synonyms: Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

Gene Symbol: SPTBN2

Poids moléculaire: 246 kDa

ID gène: 6712

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis