SOX4 anticorps (CF®640R)

N° du produit ABIN7660313

L’anticorps Souris Monoclonal anti-SOX4 a été validé pour IHC et IHC (fp). Il convient pour détecter SOX4 dans des échantillons de Humain.

Aperçu rapide pour SOX4 anticorps (CF®640R) (ABIN7660313)

Antigène: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOX4 est conjugé à/à la CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: SOX4-2540

Détail du produit anti-SOX4 anticorps (CF®640R)

Fonction: SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) (SOX4/2540), CF640R conjugate

Attributs du produit: SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-β-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival in vitro and for primary tumor growth and metastasis in vivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human SOX4 protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Breast or Colon Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur SOX4

Antigène: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Autre désignation: SOX4

Sujet:

Synonyms: SOX, SRY (sex determining region Y) box 4, SRY box containing gene 4, SRY related HMG box gene 4, Transcription factor SOX-4

Gene Symbol: SOX4

Poids moléculaire: 47 kDa

ID gène: 6659

UniProt: Q06945