TGFA anticorps (C-Term) (CF®740)

N° du produit ABIN7661056

Cet anticorps Souris Monoclonal détecte spécifiquement TGFA dans IHC et IHC (fp). Il présente une réactivité envers Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour TGFA anticorps (C-Term) (CF®740) (ABIN7661056)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MF9

Détail du produit anti-TGFA anticorps (CF®740)

Épitope: C-Term

Fonction: TGFalpha(MF9), CF740 conjugate

Attributs du produit: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF?.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Jurkat or Ramos cells. Testicular Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF-Alpha

Sujet:

Synonyms: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Gene Symbol: TGFA

Poids moléculaire: 6 kDa

ID gène: 7039

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway