Recombinant Caspase 3 anticorps (Center)
Aperçu rapide pour Recombinant Caspase 3 anticorps (Center) (ABIN7826109)
Antigène
Voir toutes Caspase 3 (CASP3) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- Center
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Fonction
- Caspase-3 antibody (HMV307)
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Immunogène
- Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Stomach: At least a moderate caspase-3 positivity should be seen in surface epithelial cells.
Product Description/Background: Caspase-3 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Stomach: Deep gastric glands and muscular cells must be caspase-3 negative.
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Protocole
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Date de péremption
- 24 months
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- Caspase 3 (CASP3)
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Autre désignation
- Caspase-3
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Sujet
- Gene Alias: caspase 3,CPP32,CPP32B,SCA-1
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UniProt
- P42574
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Pathways
- Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Antigène
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