LAMP1 anticorps (N-Term) (Alexa Fluor 647)

N° du produit ABIN7835604

Cet anticorps Rat Monoclonal détecte spécifiquement LAMP1 dans FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour LAMP1 anticorps (N-Term) (Alexa Fluor 647) (ABIN7835604)

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP1 est conjugé à/à la Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1D4B

Détail du produit anti-LAMP1 anticorps (Alexa Fluor 647)

Épitope: N-Term

Fonction: Rat monoclonal antibody AF647 labled to CD107a

Specificité: Recognizes mouse CD107a

Purification: The antibody was purified by affinity chromatography.

Immunogène: This monoclonal antibody was raised against NIH/3T3 mouse embryo fobroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: LAMP1

Sujet: Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a

ID gène: 16783

Pathways: Autophagy