LAMP1 anticorps (Pacific Blue)

N° du produit ABIN7835608

L’anticorps anti-LAMP1 Monoclonal Souris est utilisé pour la détection de LAMP1 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour LAMP1 anticorps (Pacific Blue) (ABIN7835608)

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP1 est conjugé à/à la Pacific Blue

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H4A3

Détail du produit anti-LAMP1 anticorps (Pacific Blue)

Fonction: Mouse monoclonal antibody Pacific Blue labled to CD107a

Specificité: Recognizes human CD107a

Purification: The antibody was purified by affinity chromatography.

Immunogène: Human adult adherent peripheral blood cells

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: LAMP1

Sujet: Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a

ID gène: 3916

UniProt: P11279

Pathways: Autophagy