IL3RA anticorps (PE-Cy7)

N° du produit ABIN7835704

L’anticorps anti-IL3RA Monoclonal Souris est utilisé pour la détection de IL3RA dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour IL3RA anticorps (PE-Cy7) (ABIN7835704)

Antigène: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL3RA est conjugé à/à la PE-Cy7

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6H6

Détail du produit anti-IL3RA anticorps (PE-Cy7)

Fonction: Mouse monoclonal antibody PE/Cy7 labled to CD123

Specificité: Recognizes human CD123

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD123.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur IL3RA

Antigène: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Autre désignation: IL3RA

Sujet: IL3R, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, CD antigen CD123

ID gène: 3563

UniProt: P26951

Pathways: Signalistation JAK/STAT