CTLA4 anticorps

N° du produit ABIN7835784

Cet anticorps Golden Syrian Hamster Monoclonal détecte spécifiquement CTLA4 dans FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour CTLA4 anticorps (ABIN7835784)

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivité: Souris

Hôte: Golden Syrian Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp CTLA4 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 9H10

Détail du produit anti-CTLA4 anticorps

Fonction: Armenian Hamster monoclonal antibody to CD152

Specificité: Recognizes mouse CD152

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse CTLA-4-mouse IgG2a fusion protein

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-Syrian Hamster IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CTLA4

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Autre désignation: CTLA4

Sujet: CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152

ID gène: 12477

Pathways: Cancer Immune Checkpoints