CD19 anticorps (PE-Cy5)

N° du produit ABIN7835895

Cet anticorps Souris Monoclonal détecte spécifiquement CD19 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD19 anticorps (PE-Cy5) (ABIN7835895)

Antigène: CD19 (CD19 Molecule (CD19))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD19 est conjugé à/à la PE-Cy5

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SJ25-C1

Détail du produit anti-CD19 anticorps (PE-Cy5)

Fonction: Mouse monoclonal antibody PE/Cy5 labled to CD19

Specificité: Recognizes human CD19

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD19.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD19

Antigène: CD19 (CD19 Molecule (CD19))

Autre désignation: CD19

Sujet: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD antigen CD19

ID gène: 930

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway