CD4 anticorps (Pacific Blue)

N° du produit ABIN7836272

Cet anticorps anti-CD4 Monoclonal Rat conjugué à Pacific Blue (Clone GK1-5) (ABIN7836272) détecte spécifiquement CD4 dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour CD4 anticorps (Pacific Blue) (ABIN7836272)

Antigène: CD4

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est conjugé à/à la Pacific Blue

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: GK1-5

Détail du produit anti-CD4 anticorps (Pacific Blue)

Fonction: Rat monoclonal antibody Pacific Blue labled to CD4

Specificité: Recognizes mouse CD4

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified mouse CD4.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD antigen CD4

ID gène: 12504

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events