CD47 anticorps (FITC)
Aperçu rapide pour CD47 anticorps (FITC) (ABIN7836400)
Antigène
Voir toutes CD47 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- Rat monoclonal antibody FITC labled to CD47
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Specificité
- Recognizes mouse CD47
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Purification
- The antibody was purified by affinity chromatography.
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anti-CD47 (CD47) antibody
CD47 Reactivité: Humain FACS, WB, IF, Func Hôte: Souris Monoclonal B6H12-2 unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 Reactivité: Humain FACS, IHC, IF, ELISA Hôte: Souris Monoclonal CD47-3019 unconjugated
anti-CD47 (CD47) (AA 20-140) antibodyCD47 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-219) antibodyCD47 Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 Reactivité: Humain WB, IHC, ELISA Hôte: Souris Monoclonal CD47-2937 unconjugated
anti-CD47 (CD47) (AA 241-323) antibodyCD47 Reactivité: Souris, Rat FACS, WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-CD47 (CD47) antibody (FITC)CD47 Reactivité: Humain, Porc, Non-Human Primate FACS Hôte: Souris Monoclonal MEM-122 FITC
anti-CD47 (CD47) antibodyCD47 Reactivité: Humain, Porc, Non-Human Primate FACS, WB, IHC (fro) Hôte: Souris Monoclonal MEM-122 unconjugated
anti-CD47 (CD47) (AA 19-142) antibodyCD47 Reactivité: Humain WB, IHC, ICC Hôte: Lapin Monoclonal BLR131H unconjugated Recombinant Antibody
anti-CD47 (CD47) antibodyCD47 Reactivité: Humain WB, IHC, IF, ELISA Hôte: Lapin Monoclonal 4G11 unconjugated Recombinant Antibody
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Indications d'application
- Flow: 10 μL / assay
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Protocole
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Shipped and store at 4°C for one year. Do not freeze.
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- CD47
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Autre désignation
- CD47
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Sujet
- MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47
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ID gène
- 16423
Antigène
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