CD47 anticorps (PE)

N° du produit ABIN7836401

Cet anticorps anti-CD47 Monoclonal Souris conjugué à PE (Clone CC2C6D4) (ABIN7836401) détecte spécifiquement CD47 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD47 anticorps (PE) (ABIN7836401)

Antigène: CD47

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: CC2C6D4

Détail du produit anti-CD47 anticorps (PE)

Fonction: Mouse monoclonal antibody PE labled to CD47

Specificité: Recognizes human CD47

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD47.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD47

Antigène: CD47

Autre désignation: CD47

Sujet: MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD antigen CD47

ID gène: 961

UniProt: Q08722