ITGA6 anticorps (FITC)

N° du produit ABIN7836426

Cet anticorps Souris Monoclonal détecte spécifiquement ITGA6 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ITGA6 anticorps (FITC) (ABIN7836426)

Antigène: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA6 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 4F10

Détail du produit anti-ITGA6 anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to CD49f

Specificité: Recognizes human CD49f

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD49f.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur ITGA6

Antigène: ITGA6 (Integrin, alpha 6 (ITGA6))

Autre désignation: ITGA6

Sujet: Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD antigen CD49f)

ID gène: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex