Recombinant Cyclin A anticorps (N-Term)

N° du produit ABIN7865341

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin A dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Cyclin A anticorps (N-Term) (ABIN7865341)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: R6E6

Détail du produit anti-Cyclin A anticorps

Épitope: N-Term

Expression System: CHO Cells

Fonction: Recombinant Cyclin A2 / CCNA2 (Marker of Tumor Proliferation) Antibody

Specificité: It recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogène: Recombinant protein corresponding to the N-terminal fragment of cyclin A protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: Cyclin A2

Sujet:

CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

K-562 or HeLa cells. Human tonsil or lymph node. EL4.

Poids moléculaire: 54kDa

ID gène: 890, 58974

UniProt: P20248

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA