Recombinant CDKN1B anticorps

N° du produit ABIN7865346

Cet anticorps anti-CDKN1B Monoclonal Souris (Clone r1B4) (ABIN7865346) détecte spécifiquement CDKN1B dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant CDKN1B anticorps (ABIN7865346)

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: R1B4

Détail du produit anti-CDKN1B anticorps

Expression System: CHO Cells

Fonction: Recombinant p27Kip1 (Mitotic Inhibitor / Suppressor Protein) Antibody

Specificité: P27 protein, also known as kinase inhibitory protein 1 (Kip1), is a polypeptide which binds to cyclin E/cdk2 complexes (but not to cdk2 alone) and is detected in purified extracts of growth-arrested cells. p27 protein constrains cell proliferation by setting the threshold level of cyclin E necessary to activate cdk2. The 27kD protein is also present in proliferating cells but only in a sequestered form when it is unavailable to interact with cyclin E/cdk2 complexes. It is likely that cyclin D complexed with catalytically inactive cdk4 is sufficient to sequester p27 and titrate its function. The presence of bound p27 in proliferating cells suggests that its role may not be restricted to inducing cell cycle arrest but to also set the cyclin E threshold for execution of the G1 to S phase transition during each mitotic cycle.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogène: Prokaryotic recombinant antigen corresponding to the full length p27 molecule

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: p27Kip1

Sujet:

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, KIP1, MEN1B, MEN4

ZR75, MCF7, MDA-MB-231 or SK-BR-3 cells. Human placenta,Brain, tonsil or spleen.

Poids moléculaire: 25-26kDa

ID gène: 1027, 238990

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy