MCM5 anticorps (AA 500-734)

N° du produit ABIN7865543

L’anticorps anti-MCM5 Monoclonal Souris est utilisé pour la détection de MCM5 dans des échantillons de Humain. Il a été validé pour WB et IHC.

Aperçu rapide pour MCM5 anticorps (AA 500-734) (ABIN7865543)

Antigène: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MCM5-3200

Détail du produit anti-MCM5 anticorps

Épitope: AA 500-734

Fonction: MCM5 (Transcription Factor) Antibody

Specificité: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double- stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-≥-induced Stat1± transcription activation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A.

Immunogène: Recombinant fragment (around aa500-734) of human MCM5 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur MCM5

Antigène: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Autre désignation: MCM5

Sujet:

CDC46, MGORS8, P1-CDC46

Human tonsil, spleen or lymph node, HeLa, Jurkat

Poids moléculaire: 90kDa

ID gène: 4174

UniProt: P33992

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA