RENT1/UPF1 anticorps

N° du produit ABIN7865540

Cet anticorps Souris Monoclonal détecte spécifiquement RENT1/UPF1 dans ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour RENT1/UPF1 anticorps (ABIN7865540)

Antigène: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RENT1/UPF1 est non-conjugé

Application: ELISA

Clone: NY1-1-1B6

Détail du produit anti-RENT1/UPF1 anticorps

Fonction: RENT1 / hUPF1 Antibody

Specificité: In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent1 and Rent2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A.

Immunogène: Recombinant human UPF1 protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur RENT1/UPF1

Antigène: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Autre désignation: RENT1

Sujet:

KIAA0221, RENT1, UPF1, UTF, smg-2, pNORF1

HeLa cells.

Poids moléculaire: 124kDa

ID gène: 5976

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome