Caspase 3 anticorps (AA 1-200)

N° du produit ABIN7858374

L’anticorps anti-Caspase 3 Monoclonal Souris est utilisé pour la détection de Caspase 3 dans des échantillons de Humain. Il a été validé pour IHC.

Aperçu rapide pour Caspase 3 anticorps (AA 1-200) (ABIN7858374)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: CASP3-8446

Détail du produit anti-Caspase 3 anticorps

Épitope: AA 1-200

Fonction: Caspase 3 (Pro & Active Form) Antibody

Specificité: This MAb recognizes a protein of 32 kDa, identified as Caspase 3. It is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp 175-Ser 176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp 28-Ser 29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 sub- units. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at a Asp 216-Gly 217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa1-200) of human CASP3 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase 3

Sujet:

APOPAIN, Apoptosis related cysteine peptidase, CASP3, Caspase3, CPP32, CPP32B, cysteine protease CPP32, PARP cleavage protease, Procaspase3, SREBP cleavage activity 1 (SCA1), Yama

Heart|HL60 or Jurkat cells. Human liver|liver or kidney.|spleen

Poids moléculaire: 32kDa

ID gène: 836, 141125

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation