CD74 anticorps

N° du produit ABIN7865650

Cet anticorps anti-CD74 Monoclonal Souris (Clone LN-2) (ABIN7865650) détecte spécifiquement CD74 dans FACS, IHC, IF et ICC. L’anticorps est réactif avec des échantillons de Humain, Souris et Singe.

Aperçu rapide pour CD74 anticorps (ABIN7865650)

Antigène: CD74 (HLA-DR-gamma (CD74))

Reactivité: Humain, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD74 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: LN-2

Détail du produit anti-CD74 anticorps

Fonction: anti-CD74 mouse monoclonal, LN-2, purified

Réactivité croisée (Details): negative with rat

Purification: affinity chromatography

Immunogène: cell extracts from diffuse histiocytic lymphoma SU-DHL-4

Isotype: IgG1 kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur CD74

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet: LN-2 is directed against the CD74 cluster, established during the IVth Leukocyte Typing Workshops. CD74 comprises four species of proteins (MW 41/35/33 kDa), all coded by a single gene, consisting of nine exons. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. In tissue sections anti CD74 show a binding pattern very similar to that of anti-HLA-DR. It binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. Positive control: Daudi or Raji cells. Tonsil or lymp node.

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints